ABSTRACT
To study the regulation effect of Sheng-mai San (SMS)on intestinal dysbacteriosis,ceftriaxone sodium was used to induce intestinal dysbacteriosis in vitro,the bacteria were cultured in the selective medium and enu-merated by plat counting method,and the short chain fatty acid were also analyzed .The results showed that SMS (16.5,11.2,5.5 mg/mL)could significantly inhibit the proliferation of potentially harmful bacteria such as Enterococci,Enterobacteriaceae and promote the proliferation of Lactobacillus and Clostridum.Compared with the model group,the proliferation of Enterococci and Enterobacteriaceae was inhibited by 24.9% and 19.1%,while the proliferation of Lactobacillus and Clostridum was increased by 22.3% and 25.8% respectively in the middle-concentration of SMS group (11.2 mg/mL)at 24 h.Meanwhile,SMS in different concentration significantly increase the accumulation of acetic acid and propionic acid generated by gut microbes which could significantly inhibit the proliferation of harmful bacteria.And SMS showed prebiotic effects on intestinal microflora imbalance induced by antibiotics in vitro.
ABSTRACT
Objective To investigate the mechanism of AS2 O3 inducing the apoptosis of myelodysplastic syndrome(MDS) cell line SKM-1 .Methods SKM-1 cells were incubated with AS2 O3 ,and then the cellular morphology was observed ,flow cytometry was used to determine the apoptosis ,RT-PCR was used to detect the expressions of Bcl-2 ,Bax and caspase-3 mRNA .Results 0 .25、0 .50 μmol/L AS2 O3 could not markedly induce the apoptosis of SKM-1 cells (P>0 .05) .But 2 .00、8 .00、32 .00 μmol/L of AS2 O3 could obviously promote the apoptosis of SKM-1 cells .With the increase of the acting time and concentration of AS2 O3 ,the apoptosis rate increased ,too(P<0 .01) ,the expressions of anti-apoptotic gene Bcl-2 mRNA decreased (P<0 .01) ,the expressions of promoting apoptosis gene Bax and caspase-3 mRNA increased (P<0 .01 ,P<0 .05) .Conclusion 2 .00、8 .00、32 .00 μmol/L of AS2O3 may promote the apoptosis of SKM-1 cells through down-regulating the expression of Bcl-2 gene and up-regulating the ex-pressions of Bax and caspase-3 genes .
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AIM: To investigate the effect of curcumin on the binding activity of hepatic nuclear factor-kappa B(NF-?B) and the expression of peroxisome proliferator-activated receptor-?(PPAR-?) in rats with liver fibrosis induced by carbon tetrachoride. METHODS: A total of 60 clean male rats were randomly and averagely divided into group A,B,C,D,E and F.The rats in group A served as normal controls,while those in other five groups were injected subcutaneously 40% CCI_4 for seven weeks to induce the model of liver fibrosis.After seven weeks,the rats in group C,D,E,F were intragastrically administered with 50 mg/kg silibinin,100 mg/kg cur,200 mg/kg cur,400 mg/kg cur once per day for six weeks,respectively.HE staining was used to observe the pathological changes of liver tissues under light microscope,and immunohistochemistry and reverse transcriptase polymerase chain reaction(RT-PCR) were performed to detect the activity of NF-?B,PPAR-? and mRNA expression of PPAR-?.RESULTS: The inflammatory and fibrotic degrees were obviously alleviated in group C,D,and E compared with group B.The expression of NF-?B p65 was significantly decreased in liver tissue in group C,D and E,compared with model control group B(P
ABSTRACT
Distribution of 〔14C〕 bimolane in mice was studied by means of whole body autoradiography. At 30min, and lh, 3h, 9 h, 24h after iv, the mice were briefly anesthetized with ether and frozen by immersing indry ice/acetone. The mice were processed for whole body auto-radiography by continual sections with LKB-2250 PMV big whole body automatic-manual frozen cutter. The exeriments showed that 〔24C〕 bimolane distributed rapidly into the various tissues after iv administrtion. The highest radioactivity was found in lung, kidney, gastrointestinal tract, then the tumor, skin etc, the lowest radioactivity was found in brain.